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puromycin selectable marker gene  (TaKaRa)


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    Structured Review

    TaKaRa puromycin selectable marker gene
    Puromycin Selectable Marker Gene, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 3504 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/puromycin+selectable+marker+gene/Puromycin/bio_rxiv__2025__06__13__659552-147-33-49
    Average 96 stars, based on 3504 article reviews
    puromycin selectable marker gene - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Retroviral:

    Article Title: The nucleus forms a dynamic contact with the plasma membrane to maintain the glandular epithelial architecture
    Article Snippet: .. It was used to make retroviral particles in 293T cells and to express N-terminal EGFP fusion protein in cells. pLKO2-hygro H1 lentiviral vector was derived from TRC2-pLKO-puro (Millipore-Sigma, SHC201) plasmid by replacing the puromycin selectable marker gene and human U6 promotor with a hygromycin selectable marker gene from pMSCV-hygro (Clontech) and human H1 promoter from pSUPER.retro.puro (Oligoengine), respectively. ..

    Derivative Assay:

    Article Title: The nucleus forms a dynamic contact with the plasma membrane to maintain the glandular epithelial architecture
    Article Snippet: .. It was used to make retroviral particles in 293T cells and to express N-terminal EGFP fusion protein in cells. pLKO2-hygro H1 lentiviral vector was derived from TRC2-pLKO-puro (Millipore-Sigma, SHC201) plasmid by replacing the puromycin selectable marker gene and human U6 promotor with a hygromycin selectable marker gene from pMSCV-hygro (Clontech) and human H1 promoter from pSUPER.retro.puro (Oligoengine), respectively. ..

    Plasmid Preparation:

    Article Title: The nucleus forms a dynamic contact with the plasma membrane to maintain the glandular epithelial architecture
    Article Snippet: .. It was used to make retroviral particles in 293T cells and to express N-terminal EGFP fusion protein in cells. pLKO2-hygro H1 lentiviral vector was derived from TRC2-pLKO-puro (Millipore-Sigma, SHC201) plasmid by replacing the puromycin selectable marker gene and human U6 promotor with a hygromycin selectable marker gene from pMSCV-hygro (Clontech) and human H1 promoter from pSUPER.retro.puro (Oligoengine), respectively. ..

    Marker:

    Article Title: The nucleus forms a dynamic contact with the plasma membrane to maintain the glandular epithelial architecture
    Article Snippet: .. It was used to make retroviral particles in 293T cells and to express N-terminal EGFP fusion protein in cells. pLKO2-hygro H1 lentiviral vector was derived from TRC2-pLKO-puro (Millipore-Sigma, SHC201) plasmid by replacing the puromycin selectable marker gene and human U6 promotor with a hygromycin selectable marker gene from pMSCV-hygro (Clontech) and human H1 promoter from pSUPER.retro.puro (Oligoengine), respectively. ..

    other:

    Article Title: The nucleus activates mechano-responsiveness via FHOD-associated LINC complexes
    Article Snippet: This plasmid was used to make lentiviral particles in 293T cells and to express doxycycline inducible C-terminal EGFP-tagged protein with shRNA in mammalian cells. pInducer250 tet-on EGFP-N4 was derived from pInducer200 (puro) tet-on EGFP-C4 by deleting the IRES-puro sequence and moving the multiple cloning site at the 3’ end of EGFP to the 5’ end. pInducer210 (blast) tet-on mScarlet-I-N4 lentiviral vector was derived from pInducer20 plasmid by: 1) replacing the tetracycline inducible minimal CMV promoter with the TRE3G promoter, 2) inserting mScarlet-I cDNA from pmScarlet-i_C1 plasmid (Addgene #85044) downstream of the TRE3G promoter, 3) replacing the UbC promoter with the human PGK promoter, and 4) replacing the neomycin selection marker cDNA with a blasticidin selection maker cDNA from pCW-Cas9-Blast plasmid. pLKO2-hygro H1 lentiviral vector was derived from TRC2-pLKO-puro (Millipore-Sigma, SHC201) plasmid by replacing the puromycin selectable marker gene and human U6 promotor with the hygromycin selectable marker gene from pMSCV-hygro (Clontech) and the human H1 promoter from pSUPER.retro.puro (Oligoengine), respectively.



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